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  • ISSN: 2333-6668
    Volume 4, Issue 1
    Research Article
    Abubakar Salisu* and Saba Jonathan Jiya
    The study accesses the responses of pre-sowing treatment on germination and also takes a critical look at the seedling characteristics and developmental pattern of formation and transition of juvenile to adult leaves in V. doniana. A total of 1800 fruits from 15 trees were collected from parts of North Central Nigeria (FCT and Niger State). Randomized Complete Block Design (RCBD) was adopted for the study and there were six sub treatments from two main treatments; namely chemical and hot water treatments. The seeds were subjected in to chemicals and hot water treatments at various degrees in order to speedy up the germination and break the dormancy of the fruit. The germination was recorded daily from the date of sowing and continued till the germination ceased. Chi-square (X2) test showed no significant difference between the treatments and the locations. The Least Significant Difference (LSD) showed no statistical significant of area of first pairs of leaf sizes across the three locations while LSD showed significant of petiole lengths between the three location. It was recorded that generally compound leaf formation ranges from two leaflets (bifoliolate) to five leaflets (pentafoliolate), the result shows that 50% of the plants selected for the study transited from simple to pentafoliolate compound leaf. Similarly, transition stage was between 7th and 8th leaf stage with majority (65%) of transition at 8th leaf stage. However, the study revealed that after interruption of already formed compound leaves i.e. cutting off the main apexes, majority (57%) of the seedlings reformed to simple leaves; hence, different leaf reformations were recorded.
    RK Ganapati*, R Alam, and MM Rahman
    Sugarcane is a vegetative propagated crop and hence the production of seed and its fate in the environment has little been studied. This study contributes to understanding by defining the abiotic limits for sugarcane seed germination. Using seed from multiple genetic crosses, germination was measured under different substrate regimes (Medium, Soil with Cow dung, Sand and Paper) and temperatures (25 °C and 30 to 39 °C); cardinal temperatures and suitable medium for germination were estimated based on the rates of germination. We found that sugarcane seed could germinate over a broad range of temperatures with optimum ranging from 30°C to 39 °C depending on source of seed. Beside this sugarcane true seed germinate well in paper substrate followed by sand substrate. Regarding germination among four progeny field cross (I 33-97 x I 24-07) showed better.
    Soumitra Nath*, Priyanka Yumnam, and Bibhas Deb
    Secondary metabolites produced by different plants might inhibit or stimulate the growth and development of other plants present in its vicinity, this kind of interaction among plants termed as allelopathy. Lemon (Citrus limon) peels, flowers, and leaves were chosen for test extract preparation to check the seed germination and seedling growth of lettuce (Lactuca sativa L.) and cabbage (Brassica oleracea L. var. capitata L. f. alba D C.) and to observe their allelopathic potential. The experiment was conducted in sterilized Petri dish; germination of seeds was observed for ten days and the shoot length, root length and number of germinated seeds was evaluated. The effects of different concentrations of aqueous extract were compared to distilled water (control). The results showed maximum seed germination in control whereas all the test extracts showed very low germination percentage except lemon flower extract on cabbage seeds showing 80% seed germination. Root and shoot lengths were also reduced in all the test extracts except lemon flower extract against cabbage seeds which showed stimulated shoot growth. It may be concluded that lemon plant produces allelotoxins that can inhibit the growth of other crops in its vicinity. Although lemon flower extract showed the stimulatory effect on cabbage seeds, the detailed confirmatory study is necessary.
    Mahbubur Rahaman*
    The experiment was conducted at the Laboratory of Breeding Division of Bangladesh Sugarcane Research Institute (BSRI), Ishurdi, Pabna during the period of 2011 for the effect of BA and NNA for shoot induction in vitro culture of sugarcane. The regeneration of sugarcane varieties with supplementation of 1mg/l of BA and different concentration of NNA (0.5, 1.0 and 2.0 mg/l) were used in this experiment for shoot induction. In varietal response to application of BA and NNA, among five varieties, the early days to shoot initiation were found in Isd 2-54 and Isd 40. The highest number of shoots per culture and the highest shoot length were observed in Isd 40 and Isd 37 respectively.The effect of concentrations of BA and NAA, 1.0 mg/l BA and 0.5 mg/l of NAA were showed the highest number of shoot per culture and the highest shoot length. Regarding interaction effect of varieties and the doses of BA and NNA, 1 mg/l of BA and 0.50 mg/l of NAA produced the highest number of shoots per culture and shoot length in Isd 40 and Isd 37 respectively.
    Short Communication
    Patricia Perez Galende, Candido Garcia de Maria, Juan B. Arellano*, Manuel G. Roig and Valery L. Shnyrov
    Peroxidases (EC 1.11.1.7) are a large group of enzymes widely distributed in the plant kingdom. The present work describes a study on the isolation, purification and some features of a novel peroxidase from white Spanish broom (Cytisus multiflorus), a tree legume very abundant in the northern half of Spain and Portugal. Optimal conditions are proposed for enzyme extraction, removal of phenolic compounds and enzyme purification by consecutive hydrophobic, ion-exchange and size-exclusion chromatographies. Peroxidase from Cytisus multiflorus (CMP) was found to have a molecular weight of 49 kDa. The spectrum of CMP showed a Soret band at 403 nm with a Rz factor of 3.3. Substrate specificity and the effect of some variables on the activity of CMP with guaiacol as cosubstrate have also been investigated.
    Research Article
    Rahman MM, Ivy NA, MA K Mian, Rasul MG, and Hossain MM
    An experiment conducted at Bangladesh Sugarcane Research Institute (BSRI) farm, Ishurdi, Pabna during the cropping season of 2011-2012 and 2012-2013 to evaluation of somaclonal variation under field condition. Induction of somaclonal variation and genetic transformation were used to create new genetic variability for improvement of sugarcane. One hundred twenty somaclones from each of the varieties were established and maintained in the field. Among them four somaclones from each of five varieties were selected from Ro plants. After full growth, individual somaclones were selected for agro-morphological variability, yield and yield contributing and biochemical characters. Field evaluation of somaclones and their respective parents was performed based on analysis of qualitative, quantitative and biochemical parameters. Among the five mother parents and their 20 somaclones, the best performance for number of tiller in Isd 37SC2 (137.80×103 ha-1), number of millable cane in Isd 40SC2 (96.46×103 ha-1), yield of cane was observed in Isd 40SC4 (105.40 t ha-1), the highest single stalk weight in Isd 2-54SC2 (1.20kg), plant height in Isd 37SC2 (4.71m), stalk height in Isd 37SC2 (3.04m), stalk girth in Isd 2-54SC2 (2.57cm), number of internode in Isd 37SC2 (30.23), internode length in Isd 40SC4 (12.28cm), leaf length in Isd 2-54 SC3 (1.60m), leaf breadth in Isd 2-54SC2 (5.35cm) and number of green leaf in Isd 17SC3 (15.57). In respect of biochemical analysis the highest purity percent was recorded in Isd 17SC1 (90.20). The highest brix (22%), the highest pol % cane (15.73) and the highest recovery (12.93%) were found in Isd 17SC4. These somaclones could be forwarded for the development of commercial variety.
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