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  • ISSN: 2333-7117
    Research Article
    Maide Gokce Bekaroglu, Belkis Atasever Arslan
    Abstract: β-Thalassemia major is a genetic and severe blood disorder caused by absence of β-globin chain production. Regular blood transfusion treatments can lead to modifications in cytokine levels and lymphocyte subsets. Natural killer (NK) cells are lymphocyte subtypes that are a major part of innate immune responses against infectious pathogens and tumor cells. NK activity is critical for susceptibility of β-Thalassemia major patients against cancer and bacterial infections. This review discusses recent literature focused on this issue. As independent from regular blood transfusions and iron overload, effects of the other cellular and transcriptional factors causing decreased NK activity in β-Thalassemia major patients are still unknown. If NK activity mechanisms and its abnormalities in β-Thalassemia major patients were fully understood, activity rate can be regulated to reduce the risk of cancer and infectious diseases.
    Hsi-Jien Chen1*, Han-Ja Chang1 and Meng-Shiou Lee2*
    Abstract: The objective of this study is to isolate indigenous cellulose-degrading bacteria from soil samples collected in Taiwan. After isolation by culture enrichment followed by strain acclimation, the characteristics of these bacteria were investigated. Three distinct soil samples were collected; those were hot spring drainage soil, soil from Yang-Ming Shan National Park and gas station drainage soil. Screening used washed raw cellulose as the sole carbon source and the bacteria were isolated on the 10th subcultures. The 16S rDNA sequences of the strains were compared to the NCBI and Applied Biosystems databases. Five strains distinct strain were obtained and identified as Sphingomonas sp., Pseudomonas sp. M1, Achromobacter sp., Pseudomonas sp. M2, and Stenotrophomonas sp. Growth kinetics analysis of the strains shown Pseudomonas sp. M1 gave the fastest growth rate of 0.439 h-1 with a saturation coefficient (Ks) of 766 mg CMC/L and a growth inhibition coefficient (Ki) of >200,000 mg CMC/L. The highest reduction sugar generation ratio was found with 8 g/L CMC as the sole carbon source. Pseudomonas sp. M1 had the most optimal acid resistance ability of the five strains and was able to grow in an acid environment with a pH of >3.
    Xiaobo Huang*, Chan Wang, Chengliang Chang, Ruiqiang Hang, Naiming Lin
    Abstract: The purpose of this study was to evaluate the effect of hydrogel charge affected the behaviors of hepatocytes in vitro. The hydrogel charge was adjusted by tuning the cross-linking between the alginate (Alg) and the poly-L-lysine (PLL). HepG2, a human hepatocyte cell line, was conducted as the cell model. The cellular behaviors on hydrogels were measured by cell morphology observation, viability assay, gene and protein expression analysis. As the zeta potential changing from -3.5 mV to -10.6 mV, the corresponding cell morphology transformed from monolayer cultures into aggregates. In addition, the cell behavior was also well correlated with the adsorbed fibronectin on the hydrogel surfaces. It indicated that the surface charges influenced the adsorption of fibrinogen, which might further mediate the behaviors of HepG2 cells. Therefore, hydrogel charges might be used to manipulate hepatocyte behavior in vitro to promote liver tissue engineering research and applications.
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