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  • ISSN: 2333-6633
    Current Issue
    Volume 4, Issue 2
    Research Article
    Min Qin, Li Jiang, Qian Li, Fanping Meng, and Weizhi Zhou*
    Wangia profunda SM-A87 (SM-A87), a marine bacterium, can produce massive exopolysaccharides (EPS), which possess excellent adsorption ability to heavy metals. Adsorption performance of Pb (II) ions using calcium alginate immobilized EPS (IEPS) was evaluated through batch experiments and fixed-bed column experiments. The batch experiments results indicated that the optimum conditions for adsorption were given as follows: pH = 4.0, adsorbent dosage of 0.2 g/L, contract time of 480 min, temperature 20°C . The adsorption isotherm data were well fitted with Langmuir model, the adsorption kinetics could be well expressed by pseudo-second-order model, and IEPS showed good reusability in the adsorption of Pb (II) ions. The fixed-bed column results showed that a decrease in flow rate and an increase in bed height caused an increase in the breakthrough time with enhanced Pb (II) ions removal capacity. The adsorption isotherm data were well described with the Thomas model. This study demonstrates IEPS could be an environment friendly and effective bio-adsorbent for heavy metal ions removal in wastewater.
    Chen Wang*, Isabelle Bourven, Michel Baudu, and Abdel Lakel
    Extracellular polymeric substances (EPS) from biofilters (packed with river sand (RS) and crushed aggregate (CA)) set in on-site wastewater treatment systems are characterized over 360 days within bed thickness to investigate its correlation with the enrichment process. Biochemical component contents are monitored. Moreover, humic and protein-like compounds are characterized by means of Size Exclusion Chromatography (SEC) coupled with fluorescence. During the biomass enrichment phase, EPS biochemical components increase at the top of the biofilter (protein enrichment factor >70%). The protein-like components exhibit a very high MW fraction (apparent molecular weight (aMW) >1,000kDa), which may contribute to cell aggregation. Humic-like substances show similar SEC fingerprints to those of the feed water (aMW<6kDa) and are perhaps being metabolized at around Day 210 (as evidenced by a lower aMW). Only the dynamic polysaccharide partition in EPS differs between biofilters, with an increase for CA and a decrease for RS. Within the filtration bed thickness, lower biomass with a higher EPS content is observed, and the polysaccharide fraction increases by a factor of 2. Protein-like components exhibit a very high MW fraction at a lower magnitude. The clogging risk due to the presence of polysaccharides or their combination with high aMW extracellular proteins should be considered for alternative materials for onsite wastewater filtration system.
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