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  • ISSN: 2333-6668
    Current Issue
    Volume 6, Issue 4
    Research Article
    Swe Zin Yu, Nwe Ni Win Htet, Thet Su Hlaing, and San San Yu*
    Since recent years, bioethanol has become greatly interested as an alternative to petroleum derived fuel. The recent study aims at searching for effective native yeast strains for bioethanol production. In this study, fourteen yeast strains that were collected from different sources were identified based on carbon and nitrogen assimilation tests and fermentative capacity tests. Among them, five yeast strains could be assumed as Saccharomyces cerevisiae according to the conventional identification. The ethanol and temperature tolerant tests were also carried out. According to ethanol and temperature tolerant tests, the isolate Y3 had the highest temperature and ethanol tolerant level (45C and 10% v/v respectively).
    Ei Phyu Kyaw*, Tin Mar Lynn, Zaw Ko Latt, and San San Yu
    In this research, twenty isolated strains of cellulolytic nitrogen-fixing bacteria were isolated from different soil sources. Cellulase activity of each selected strain was detected by screening of clear zone formation and by using DNS (Dinitrosalicylic colorimetric) method. The clear zone formation were not significant among these strains but amongst them, for cellulose substrate isolated strain K-7 was considered as the most potent isolate in term of their high cellulase productivity in the concentration of 0.452 mg/0.5 ml and in carboxymethyl cellulose substrate, M-1 strain showed maximum yield for cellulase production in the concentration of 0.493 mg/0.5ml. Nitrogen fixation activity of those selected strains was measured by the color changes in G-NFMM (Glucose-Nitrogen Free Mineral Medium) supplemented with BTB (Bromothymol Blue) and ammonium test kit. Composting was studied by using six strains on straw waste. Finished composting was observed for a month and nitrogen rich compost was obtained. The finished compost was used as carrier system and six selected bacterial strains for nitrogen fixation were used as formulated biofertilizer and applied in rice field. They improve rice plant growth, yield and nitrogen content in soil when compared with other rice plant used in chemical fertilizer. Our research can fulfill the aspect of enhancing the usage of environmentally friendly biofertilizer without reducing farmers profit by maintaining the good yield.
    Sakineh Abdi and Alireza Pirzad
    Water stress and the deficiency of mineral nutrients are major constraints that limit forage legumes production, particularly in arid and semi-arid regions. To evaluate the role of arbuscular mycorrhizal fungi (AMF) in alleviating the aforementioned condition, a greenhouse factorial experiment on sainfoin plant (Onobrychis sativa L.) was conducted. The experiment was based on the completely randomized design (CRD) with three replications in 2014. The treatments were carried out on species of fungi (Faneliformis mosseae, Rhizophagus intraradices, Claroideoglomus claroideum, Funneliformis caledonius, Glomus versiforme, and non-inoculated control) and irrigation (irrigation at 80% (well watering) and 50% (water deficit) field capacity (FC)). The highest root colonization (66%) occurred with G. versiforme in well-watered plants. The mycorrhizal (F. mosseae and Rh. intraradices) sainfoin leaf chlorophyll index (SPAD) increased as a result of water deficit stress. The leaf osmolytes (proline and total soluble sugars) increased in all mycorrhizal plants. Mycorrhizal species produced taller plants than those produced under non-inoculated control. The highest leaf phosphorus content was obtained from plants inoculated with F. mosseae (294.0 mg / 100 g dry weight). In well-watered sainfoin plants, the highest percent of leaf N (4.73%) belonged to G. versiforme. Leaf dry weight showed a significant decrease in irrigation at 50% FC, but the mycorrhizal symbiosis compensated it to more than well-watered plants. In this research work, all mycorrhizas species were significantly enhanced the leaf properties due to highly root colonization caused in water and phosphorus uptake.
    Rebaka Sultana, Ismail Hossain MD, Saifullah MD, Ruhul Amin, and Rajesh Chakraborty*
    Experiment was carried out in mushroom research shade house of Olericulture Division, HRC (Horticulture Research Centre), Bangladesh Agricultural Research Institute, Joydebpur, Gazipur, during the period from July, 2015 to December, 2015 to evaluate the effect of pH levels of substrates and frequency of watering on the growth traits of oyster mushroom. Spawn of Pleurotus ostreatus (Jacquin ex Fr.) was used as test crop. The experiment consisted of two factors i.e., pH level of substrates [P0: Control (5.5), P1: 5.0, P2: 5.3, P3: 5.8, P4: 6.1 and P5: 6.4] and frequency of watering [W0: Control (No immersion in water and no further watering), W1: Im + 12h, W2: Im + 18h, W3: Im + 24h, W4: Im + 30h and W5: Im + 36h]. The experiment was laid out in Completely Randomized Design (CRD) with three replications. Results revealed that most of the parameters showed the significant response due to different pH levels of substrate and watering frequency. For pH level of substrate, the treatment P0 (5.5) exhibited the better performance on the growth characters of mushroom. In case of watering frequency, the treatment W1 (Im + 12h) showed higher number of fruiting bodies and higher number of harvest of mushroom. Statistically, the highest number of fruiting bodies was obtained from the combined treatment P0 W1 i.e., pH of substrate 5.5 in combination with watering frequency Im + 12 h. So, this combination may be used for higher yield of mushroom.
    Behnam Tahmasebpour*, Mohsen Sabzi Nojadeh, and Mohammad Esmaeilpour
    In order to evaluate the effect of salinity stress (three treatments including control, 6% and 20% sodium chloride) on yield and components of seed yield of spring canola (Sarigol, Delgan, Zaffar, Zarfam, RGS003), a factorial experiment based on Randomized Complete Block Design with three replications was conducted. Main effects of cultivar, salinity stress and interaction of salinity stress cultivar in all evaluated traits were significant at 0.01 P-values. In all cultivars, the highest values of yield and its components were observed under non stress condition. Grain yield significantly decreased with increasing salinity levels. Dahlan cultivar (19.3 gr grain yield) had the highest seed yield under control conditions. Stepwise multiple linear regression analysis showed that grain weight per pod and number of pods per plant had the highest effect on grain yield and explained 95% of grain yield variations. Factor analysis showed that four factors explained 80.81% of total variations. The first factor explained 36.07% of total variance and was named as an effective factor in vegetation growth. The second factor explained 16.28% of the variation and was identified as the grain yield and its components factor. The third and fourth factors explained 14.8% and 9.66% of total changes respectively, and were named as root dry weight and one thousand seed weight factors respectively. In this research, Sarigolcultivar was introduced as tolerant cultivar due to the highest grain yield under salinity stress conditions.
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