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  • ISSN: 2333-6668
    Current Issue
    Volume 7, Issue 1
    Research Article
    Elif Oztetik*
    Today, heavy metal (HM) pollution is one of the most important ecological problems worldwide. Higher concentrations of HMs can lead to toxic effects in all organisms. Some HMs, such as Cd and Pb, although being non-essential and without physiological function, are very toxic even at very low concentrations, which cause some serious disruption in plant growth and productivity with a heavy losses in agricultural yield and crop production. Beside their negative impacts on plants, transfer of toxic elements to the food chain leads to several diseases which human beings faced. Therefore, to reduce the risk of contamination in human beings, HM tolerant varieties should be selected and use for phytoremediation purposes where necessary.In this study, the effects of HM stress onsome of those enzymatic and non-enzymatic antioxidant defense mechanisms, together with protein contents were investigated in two different crop varieties. The selected concentrations (0,150,300 M) of single PbCl2, CdCl2 and their combinations (PbCl2 + CdCl2) were applied in hydrophonic solution to examine the changes of glutathione (GSH), protein and glutathione S-transferase (GST) activities in the roots and shoots of Hordeum vulgare cv. Erginel and Triticum aestivum cv. Bezostaya varieties.Results indicates that, both single and combined treatments cause a difference at some extend depending on the plant, plant parts and concentrations of HMs. Observation of high levels in examined parameters according to control values indicatesa general adaptability to stress conditions.In line with our results, barley variety were found to be more tolerant to HM stress by comparing to wheat andcan be used for remediation purposes at contaminated sites as a plant agent.
    Manisha Ray, Smruti P. Pradhan, and Souvagyalaxmi Sahoo*
    SSRs or microsatellites identification and its functional analysis has a key role in different sectors of genomics such as genome organization, gene regulation, quantitative genetic variation, evolution of genes and plant breeding sectors. Therefore, computational approach was undergone for identification and analysis of SSR within functional domain of Citrus limon (C. limon) of family Rutaceae, is one of the vital and effective medicinal plant. Total 1644 numbers of extracted ESTs of C. limon were validated through Tandem repeat finder and VecScreen which have been assembled in CAP3 that resulted 55 contigs and 1183 singletons. Afterwards, total 420 SSRs were identified as SSR-ESTs using MISA tool and also detected 75.23% of mononucleotide SSR motifs with most ample sort of repeats such as di- (9.52%), tri- (7.61 %) and tetra- (0.95 %) nucleotide. Ultimately, 128 SSR sequences have been selected with appropriate primer properties which would be used as markers to look at transferability to related species. Further, the useful functional annotation was performed using Blast2GO. These findings would assist to understand the significance of SSR markers and also to facilitate the evaluation of genetic range in medicinal plant flora.
    Yohannes Dereso* and Ali Seid
    The present study is about ethnobotany and conservation issues of the multipurpose Oxytenanthera abyssinica in the Mandura Woreda. The Woreda has patchily distributed vegetation features characterized by Combrutum molle and Entada abyssinica growing in association with O.abyssinica, commonly known as lowland bamboo. The life of native people in Mandura Woreda is strongly dependant on law land bamboo for their socio-economic well binges. The lowland bamboo forest in the Woreda was flowered in 1998 E.C, but the current burning environmental issues in the Woreda is deforestation due to fuel-wood consumption, timber harvesting, farm expansion, and forest burning. O.abyssinica is multipurpose tree species in Mandura Woreda; about 20 local uses ofthe spices are identified. Pair wise ranking with 5 highly preferred woody species of different uses was conducted and O. abyssinica was first choice for construction and beehive making; and ranked fourth for pole and fire wood. The new shoots of the species also used as food during summer season, which is time of food shortage, and as Tosha (poison and alcohols antidotes). Lowland bamboo vegetation is threatened by cutting, decay of new shoots, mass flowering, agricultural expansion, over grazing, fire and cutting style of which fire is the most leading threat reported. Using a participatory method, five important methods of conservation were identified to conserve the species. They were: government measures, community forest protection, encouraging cultural by laws, cultivation and proper cutting style. Of these methods, government measure was thought central and decisive. Awareness creation is among the recommendations.
    Soubhagyalaxmi Sahoo*, Subhashree Subhasmita Raut, and Debasrita Das
    Centellaasiatica is a valuable medicinal herbaceous aromatic creeper which has been valued for centuries in ayurvedic medicine. Phytochemical analysis of Centellaasiatica plant extracts revealed the presence of various biochemical compounds such as alkaloids, flavonoids, glycosides, triterpenoids and saponins etc. Since triterpenoids and flavonoids have remarkable anti-inflammatory activity also Phyto compound possess a wide range of activities, which may help in protection against tuberculosis diseases. In this study, the molecular docking was applied to explore the binding mechanism and to correlate its docking score with the activity of plant derived compounds and also identified targets were validated through some other databases to select the better ones and design the drug. This study reveals that NOD2 is a better potential drug target for tuberculosis and Quercetin is the best ligand to inhibit NOD2 and might be prevent the disease Tuberculosis. Our result can be useful for the design and development of novel compounds having better inhibitory activity against several type of Tuberculosis. This potential agent will be a promising candidate can further be validated in research for its proper function. This work would help to design a potent lead molecule against any other disease.
    Short Communication
    Aviv Rapaport, Elazar Quinn, Avihai Harush, Moshe Kostyukovsky*, and David J. Bonfil
    Sunn pest Eurygaster integriceps Put. on is one of the most serious factors affected the gluten quality of wheat grain in Israel. The object of this study was to evaluate the damage of sunn pest to wheat quality of some cultivars of spring wheat. Field experiments were conducted in three cultivars with a different phenology: Zahir - early ripening, Bar-Nir - medium, Ruta - later ripening. The isolated by net plots were infested by unsexed wintering adults of sunn pest. The isolated uninfested plots serve as a control. The sunn pests effects on quantitative parameters of wheat quality, such as thousand kernels weight, test weight, and the effects on gluten quality and quantity, such as gluten index, IDK test, wet gluten content, were examined. In addition, samples at varying degrees of the bug-damaged kernels were constructed in order to examine the quality and quantity of gluten. A high level of bug-damaged kernels of 15-20% did not affect the thousand kernel weight and test weight of the three tested cultivars. At a very high level of bug-damaged kernels of 40-60%, the sunn pest caused a significant reduction in thousand kernels weight in Zahir and Ruta cultivars. Test weight decreased significantly in all three cultivars. A damage of up to 4% of the kernels has significantly affected gluten quality but not gluten content. The results of this study indicate that the cultivar of wheat is of high importance for the level of the gluten quality and the degree of sunn pests damages.
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