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  • ISSN: 2333-6668
    Current Issue
    Volume 6, Issue 2
    Research Article
    Orlanda Pvoa*
    Jacaranda is a deciduous tree from South America, used as an ornamental tree due to its abundant flowering, usually found in South Portugal. The tree also has forest interest.
    Fruits were harvested in 2010, 2013, 2015 and 2017 in the city of Elvas. From the samples collected in 2013 and 2017, two fruit subsamples were obtained: a) dark brown fruits, whose maturation was reached in the previous year and b) light brown fruits, whose maturation was reached in the harvest year. In 2015, 2 subsamples were also collected: a) dark brown fruits and a mixture of all fruits types. The samples were threshed, labeled and conditioned in a refrigerator (ca. 5°C). The germination assays were carried out at 20°C with 12 h of photo phase. Final observations were obtained after 21 days.
    The germination results ranged from 11.3% (dark brown, old fruits) to 93.5% (light brown, new fruits). The best results, without significant statistical difference between samples, ranged from 70 to 93.5%, indicating that the seeds of this species harvested in Alentejo (Elvas) have high germination capacity. In the fruit harvesting operations, it is important to separate the fruits, because in the 3 tested samples of seeds harvested in different years, the seeds obtained from light brown, new fruits, had higher germination rates (9 to 35% more), with significant statistical difference from dark brown, old fruits. The seeds harvested in 2010 maintained their germination capacity (71%) when stored in a refrigerator (ca. 5°C) for 7 years.
    Tofail Hosain, Elias Hossain, Rezowana Nizam, Fazle Bari ASM, and Rajesh Chakraborty*
    Higher and stable yield of the rice is the main bottleneck in Bangladesh due to the use of suboptimal management strategies in rice field. From this perspective, the experiment was carried out to investigate the impact of the system of rice intensification (SRI) on physiological characteristics and productivity of hybrid rice varieties over inbred rice cultivation under traditional system. Results demonstrated that, the SRI cultivation method was more suitable than traditional cultivation method in respect of growth and yield of hybrid rice. Among eight rice varieties, BRRI hybrid Dhan 3 exhibited the best performance in relation to its morpho-physiological characteristics and yield attributes. Consequently, the highest yield (9.77 t ha-1) was obtained from SRI BRRI hybrid Dhan 3 followed by SRI Tia (9.19 t ha-1) and SRI Heera 3 (8.46 t ha-1). Under SRI method, all the studied hybrids showed the higher yield. BRRI hybrid Dhan 3 with SRI method produced highest total dry weight hill-1 (80 g) at maturity, significantly higher number of panicles hill-1 (19.67 ), highest filled spikelets panicle-1 (204), 1000-grain weight (28.77 g) and harvest index (45.06 %) whereas under Traditional method, higher grain yield (7.85 t ha-1) was recorded from the hybrid Dhan Tia. Among hybrid and inbred varieties, BRRI Dhan 45 showed the lowest performance under traditional method in term of all studied parameters. So, SRI method was better for cultivation of the hybrid rice varieties compared to traditional method for improving the condition of farming community as a whole.
    Khaiyam MO*, Islam MS, Ganapati RK, Uddin MJ, and Hossain MI
    A field experiment was conducted in farmers field of two locations under High Ganges River Floodplain (AEZ-11) of Bangladesh during 2013-2014 cropping season for evaluating the comparative suitability regarding yield and economic performances of six newly released sugarcane varieties viz, Isd 34, Isd 36, Isd 37, Isd 38, Isd 39 and Isd 40 with the old popular variety Isd 16. The experiment was laid out in a Randomized Complete Block (RCB) design with three replications. At Chuadanga the highest cane yield of 112.54 t ha-1 was obtained in Isd 37 which is statistically similar with Isd 39 and Isd 40 and the lowest yield was obtained in Isd 34. But at Jessore the maximum yield of 110.19 t ha-1 was recorded from Isd 39 followed by Isd 37 (99.22 t ha-1) and the minimum from Isd 36. Similarly, the newly released varieties Isd 37, Isd 39 and Isd 40 gave higher economic return compared to old variety Isd 16 in High Ganges River Floodplain of Bangladesh. Therefore, these three sugarcane varieties can be cultivated in this area with recommended management practices for acquiring higher yield and economic benefit.
    San San Yu*, Matthias Ullrich
    A nitrogen-fixing bacterium Azotobacter vinelandii was isolated from the mangrove rhizospheric soil of Irrawaddy Region, Myanmar. Screening and quantitative determination of the nitrogen fixation activity and alginate producing activity of the isolated strain was studied. The algD and algU gene fragments of Azotobacter vinelandii were isolated by using designated primers. A deletion was engineered in the cloned algD and algU genes by digestion with suitable restriction endonucleases and kanamycin resistance gene cartridge was inserted. The mutation was subsequently transferred to the host bacteria, Azotobacter vinelandii by biparental mating using pEX18 vector and E .coli ST 18 under pressure of kanamycin selection. Two mutant strains were developed and confirmed by PCR. The resultant two mutant strains lost their nitrogen fixation activity due to loss of their alginate producing activity were observed.
    Kan Liu*
    The MinION of Oxford Nanopore Technology (ONT) is a portable single-molecule sequencer released in 2014. As a portable nanopore-based sequencer [1], with just a USB drive size which can be conveniently used connected to a desktop or laptop using the USB 3.0 or higher interface, MinION contains pores embedded on a membrane that is placed over an electric grid.
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