Electrochemical Determination of Nicotine in Cigarette, and Urine Using para-Aminobenzene Sulfonic Acid Grafted Screen Printed Carbon Electrode - Abstract
In this study, a simple, scalable, and low-cost electrochemically grafted screen printed carbon electrode was developed for sensitive and selective determination of nicotine. First,simple and fast activation of bare screen printed carbon electrode was performed by linear sweep voltammetry using KOH solution, then the electrode is grafted electrochemically using in-situ generated para-aminobenzene sulfonic acid diazonium salt and was used for the determination of nicotine. The voltammetric study of nicotine in Britton Robinson buffer solution pH 8.0 showed an electrocatalytic effect towards nicotine oxidation, which cause in a larger current response when matched with the bare. Under the optimized conditions,a linear calibration curve was obtained using square wave voltammetry at the para-aminobenzene sulfonic acid grafted electrode in the range of 0.5-300 µM with detection and quantification limit 0.35 µM and 1.14 µM, respectively.