Separate Effects of Ciprofloxacin, Metronidazole, or Minocycline on Microorganisms from Deep Caries - Abstract
The study was carried out to evaluate antibacterial susceptibility of bacteria from deep carious lesions to 3 different antibiotics Ciprofloxacin, Metronidazole, or Minocycline that
were tested at concentrations (0.5, 1, 2, 4, 8µmg/ml) by the disk diffusion method. Carious, infected dentin close to the pulp was collected from teeth with deep cavities and cultured
in thioglycolate and brain heart infusion broths for 48hrs anaerobically and aerobically.
The infected broths were tested for adequate growth and colony forming units against the McFarland Turbidity standard. It was then spread onto Brucella and Brain Heart
Infusion Agar plates. Disks with the different concentrations were then placed on the agar surfaces. Each concentration was tested nine times. The control group consisted of agar
plates infected with broth but without antibiotics. All agar plates were then placed in anaerobic or aerobic environments. The plates were checked for zones of inhibition after 48 hrs. All control groups had bacterial growth. A zone of inhibition was seen for all Ciprofloxacin concentrations tested under both aerobic and anaerobic conditions. Metronidazole and
Minocycline did not show any zones of inhibition. Ciprofloxacin shows promise as an antibacterial agent against bacteria from deep caries.