Effect of Casearia Sylvestris Hydroalcoholic Crude Extract on Histological Parameters for Skin Reorganization in a Pre-Clinical Model of Postoperative Wound - Abstract
The skin is the largest organ in the body, with a total area of about 2 m². It is estimated that one in Twenty-two people in the world suffers from an epithelial surgical insult, of which there is a large proportion of anomalous repairs, resulting from exacerbation of local inflammatory processes. Therapeutic strategies that minimize such conditions are of great interest, including that use of folkloric herbal medicines. Thus, the present experimental study intends to investigate the effect of CS on histological parameters of epithelial reorganization in a pre-clinical model of postoperative wound. To that end we maintained 40 male Swiss mice in controlled environmental conditions. On the first day the animals were submitted to a 5 mm plantar epithelial incision in the right hind paw, one stitch suture and 50 µg topic treatment solutions: dexamethasone 0,5 mg/mL, acetone 2,5% and hydroalcoholic crude extract of CS (HCE-CS) 30 mg/site every 24 hours for 5 days. Every day after the treatments the surgical wound were photographed and on the third and fifth day, the mice were euthanized, the hind paws were collected for histological analyzes. To compare all different treatment groups we used one-way ANOVA methodology, followed by post hoc Dunnett’s test for comparison. All animals shrank their scars on fifth day and in the group treated with HCE-CS we observed a larger dermal thickness and smaller scar proportions related to other groups on third day, showing an accelerated inflammation resolution.