Prediction of Graft Function after Liver Transplantation in Rats Based on Mitochondrial Permeability – a New Prognosis Scoring System to Identify Marginal Organs after IschemiaReperfusion Induced Graft Injury - Abstract
Aim: Transplantation of expanded donor criteria organs due to prolonged cold ischemia is one of the major options to increase the use of such organs and therefore reduces mortality. However this may lead to dysfunction of the organ. Therefore it’s necessary to develop a predictive analytic method in order to identify such expanded donor organs and exclude them from transplantation. Method: The government of Bavaria, Munich, approved the animal studies.We used special LEWIS-(RT)-rats which were divided into three groups (n=4), depending on cold ischemia time and loss of ATP production after 2, 4 and 8 hours(post explantationem). The evaluation of the ATP production and histopathology was limited to such animals where an hepatic phase was <20 minutes und the transplantation time <60 min. For statistical analysis we used a program called software R, Version 2.12.2 (Development Core Team). The significance was 5 %. Result: There was an inverse correlation between the ATP/protein date and the histopathology of the ischemia-reperfusion-injury (p=0,003). Discussion: We evaluated the degree of the ischemia-reperfusion injury by comparing the mitochondrial permeability and the histopathology. By having a prolonged ischemia of 8 hours, the evaluation of the ATP/protein production was more sensitive than the histopathology. Thus, this fact could be used in order to obtain a score that could identify extended donor organs.