Tobacco and the polymorphism of survivin gene at its promoter region are the risk factors of Oral cancer. Our aim of this study is to find the association between tobacco and survivin -31C/G polymorphism in Oral cancer. It is a case-control study with 47 cases of OSCC and 100 controls. We used polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) protocols on blood samples of patients. A statistically significant relationship between naswar and GC genotype (P value= 0.03). The GG and GC genotype also showed similar association with gutka (P value=0.0003 and 0.0031) and betel quid (P value=0.018 and 0.0041) respectively. However our study did not find any statistically significant association of Survivin genotypes with Areca nut and smoking. Our study found a positive association between Survivin -31 genotypes and Naswar, Gutka and betel quid, however smoking and areca nut didn’t show any statistically significant association with either of Survivin variants. The synergistic combination between different varieties of tobacco and survivin -31 polymorphism can aid in the development and progression of oral cancer.