Background: Accumulated research evidence suggests a link between dyslipidemia and suicidal behavior. The aim of this study was to estimate the gender characteristics of the lipid profile of parasuicidal individuals who made a suicide attempt in various ways (highly lethal and non-violent). Results: The level of total cholesterol and LDL cholesterol in both groups of male parasuicides was lower than in the control group. The level of these indicators in the blood plasma of men who made a suicide attempt using highly lethal methods and who committed non-violent parasuicide was approximately the same. There were no statistically significant differences from the control in the level of these indicators in both groups of women who committed parasuicide. Conclusions: These findings suggest that hypocholesterolemia is a biological marker of suicidal behavior in men.