This semi-experimental study was conducted to determine the effect of laughter therapy on stress and anxiety of the students. 65 fourth-year nursing students, who agreed to participate in the study, were included in the sample of the study. In the study, the data were collected using pretest and posttest technique. In the pretest, stress and anxiety score and information were obtained from the students using the personal information form. After 20-minute laughing therapy, posttest was administered and stress and anxiety scores were evaluated again. The mean age of 65 students participating in the study was 22.05 ± 1.29 [min: 20- max: 29] and 24.6% were male students. The statistical analysis revealed that laughter therapy significantly reduced anxiety [t = 7.509, p <0.001] and stress = 7.422, p <0.001]. As a result of the study, it was seen that laughter therapy was an effective approach to reduce stress and anxiety in university students.