Human induced pluripotent stem cells (iPSCs) and mesenchymal stem cells (MSCs) have considerable potential in regenerative medicine. Moreover, there are cell banks that collect stem cells for HLA-matched medical applications. In this study, using episomal plasmids vectors, we generated iPSCs that were derived from human gingival fibroblasts (HGF). The gingival tissues were collected as medical waste after dental implant operation. Transfected cells were cultured for six days in DMEM containing 10% FBS. Cells were subsequently transferred onto SNL76/7 feeder cells in human embryonic stem cell medium (DMEM/F-12 containing 20% KSR, 2 mM L-glutamine, 1% NEAA, 0.1 mM 2-ME, and 5 ng/mL bFGF). For passaging, iPSC colonies were detached with a CTK solution [70% PBS, 20% KSR and the other 10% mixture solution (2.5 µg/mL trypsin, 1 mg/mL collagenase IV and 1 mM CaCl2 / PBS)] and split at a 1:4 ratio every 5 or 6 days. iPSCs expressed pluripotent stem cell-specific markers, such as ALP, OCT3/4, NANOG, SSEA-3, SSEA-4, TRA-1-60 and TRA-1-81 as determined by histochemistry, immunochemistry and gene expression analysis. iPSCs were successfully differentiated into MSC-like cells (MSLCs) that were evaluated by flow cytometry for human mesenchymal markers such as CD44, CD73, CD90, CD105, CD34 and CD45, and their trilineage differentiation ability. In this report, we introduce a practical way to obtain MSLCs. Our culture methodologies demonstrate that iPSCs could be a promising source of readily accessible stem cells to investigate the potential of MSLCs for future clinical applications.