Urban green infrastructure generates substantial amounts of renewable plant biomass that Structural identification was confirmed through complementary analytical techniques. TLC demonstrated complete chromatographic agreement with the reference standard. IR spectra revealed characteristic absorption bands corresponding to the flavanone structure. GC–MS analysis showed a molecular ion at m/z 270 together with diagnostic fragmentation patterns, while ¹H NMR spectra confirmed the presence of the expected aromatic, methoxy, hydroxyl, and flavanone proton signals. The results also demonstrated that pinostrobin can be effectively recovered from the hexane fraction, indicating partial solubility in this non-polar solvent and providing an alternative purification strategy. The proposed isolation method contributes to the sustainable utilization of biomass derived from urban green spaces by transforming poplar buds into a valuable source of high-purity natural flavonoids. The developed approach expands opportunities for the circular use of urban plant resources and supports future applications of bioactive compounds in agriculture, pharmaceuticals, and green technologies.