Low back pain is frequently associated with disc degeneration, which is being treated through tissue engineering of the annulus fibrosus (AF). This study aimed to utilize 3D bioprinting to fabricate a chitosan-coated Polyurethane (PU)-based scaffold (PUCh) for AF tissue engineering with suitable biological, physical, and mechanical properties. PU scaffolds were fabricated by depositing PU struts at 90°/0° towards the horizontal surface. For biocompatibility purposes, PU scaffolds were coated with different content of chitosan (0, 1, 2.5, and 5 %). The scaffold morphology, surface roughness, mechanical strength, degradation, as well as biocompatibility were characterized. Overall, PUCh indicated appropriate surface roughness, elastic modulus (1.25 MPa), swelling ratio (50%), and degradation rate (3.56 %), subsequently having a high level of biocompatibility (94 %). The presence of Ch reduced the space between the struts. In conclusion, PUCh can be considered in AF tissue engineering after suitable modification.