Momordica charantia L. (Family: Cucurbitaceae) is a well known medicinal plant and widely distributed and cultivated in many parts of the world. Momordica is a powerful, nutrient-dense plant, composed of a complex array of beneficial compounds. Seeds were collected from arsenic free area and were propagated in tissue culture media in presence of different concentration of sodium arsenate along with control one. The aim of the present study was to investigate some biochemical parameters of extracts of Momordica under the influence of arsenic. Chlorophyll and carotenoid content was affected by arsenic maximum (150 µM) in Momordica. Arsenic induced alteration in free radical scavenging activity, total polyphenolic content, lipid peroxidation, antioxidant activity and flavonoid content were determined in this study.