Biogenic Derived Ag and Zno Nanoparticles Using Couroupita guianensis Aubl. Fruit Extract and its Antibacterial Potential - Abstract
In this study, we report the synthesis of Ag and ZnO Nanoparticles (NPs) produced from the fruit extract of C. guianensis. Silver (Ag) and Zinc Oxide (ZnO) have extensive applications in various areas of science and technology. These NPs were characterized using UV-Visible (UV-Vis) spectroscopy, Fourier Transform Infrared (FTIR) spectroscopy, X-Ray Diffraction (XRD) spectrum and Atomic Force Microscopy (AFM). The UV-Vis absorption spectra generated strong characteristic peaks of AgNPs (370nm) and ZnO-NPs (374nm). FTIR spectrum analysis was conducted to validate the use of phytochemicals in the reduction, capping and stabilization of Ag and ZnO-NPs. XRD spectra were examined to ensure phase purity, crystalline nature and size of the AgNPs (42nm) and ZnONPs (26nm) respectively. Finally, the ultrastructural and nanomechanical properties of the NPs were studied via AFM analysis. Besides, this study determines antibacterial efficacy of Ag and ZnO-NPs fabricated from C. guianensis fruit extract against B. subtilis and E. coli bacterial strains. The B. subtilis had highest inhibition zone activity than E. coli and it was found to be AgNPs (12.02 ± 0.32 mm) and ZnO-NPs (11.18 ± 0.34 mm) respectively. This study demonstrated a significant potential for the use of these particles in biomedical applications due to their remarkable antibacterial activity.