MYB transcription factors play a crucial regulatory role in plant growth, development, and response to abiotic stress. A cDNA sequence encoding for the MYB gene was cloned from Morus multicaulis variety YU-711. The gene has an open reading frame (ORF) of 882bp encoding 293 amino acid protein with a predicted molecular weight of 41.7 kDa and an isoelectric point (Pl) 7.61. The mulberry MYB gene is highly conserved. It contains SANT myb-like DNA-binding domain, SHAQKYF class, and belongs to the SNAT superfamily. The evolution analysis shows that the Morus multicaulis MYB of an unknown protein is closely related to the Morus notabilis. The qRT-PCR analysis revealed that the MYB gene is highly expressed in Mulberry and is regulated during abiotic stress (salt, drought, and cold). There was an increase in the mRNA transcription level under all the stress treatment performed compared to the control. The western blot analysis shows that the His-tag MYB protein was expressed in E. coli. Explaining the MYB protein and regulation that is possible in Mulberry will provide the basis for the function and predict the contribution of MYB proteins to the biology of Mulberry plant during breeding for stress resistance variety.