Since recent years, bioethanol has become greatly interested as an alternative to petroleum derived fuel. The recent study aims at searching for effective native yeast strains for bioethanol production. In this study, fourteen yeast strains that were collected from different sources were identified based on carbon and nitrogen assimilation tests and fermentative capacity tests. Among them, five yeast strains could be assumed as Saccharomyces cerevisiae according to the conventional identification. The ethanol and temperature tolerant tests were also carried out. According to ethanol and temperature tolerant tests, the isolate Y3 had the highest temperature and ethanol tolerant level (45ºC and 10% v/v respectively).