An experiment conducted at Bangladesh Sugarcane Research Institute (BSRI) farm, Ishurdi, Pabna during the cropping season of 2011-2012 and 2012-2013 to evaluation of somaclonal variation under field condition. Induction of somaclonal variation and genetic transformation were used to create new genetic variability for improvement of sugarcane. One hundred twenty somaclones from each of the varieties were established and maintained in the field. Among them four somaclones from each of five varieties were selected from Ro plants. After full growth, individual somaclones were selected for agro-morphological variability, yield and yield contributing and biochemical characters. Field evaluation of somaclones and their respective parents was performed based on analysis of qualitative, quantitative and biochemical parameters. Among the five mother parents and their 20 somaclones, the best performance for number of tiller in Isd 37SC2 (137.80×103 ha-1), number of millable cane in Isd 40SC2 (96.46×103 ha-1), yield of cane was observed in Isd 40SC4 (105.40 t ha-1), the highest single stalk weight in Isd 2-54SC2 (1.20kg), plant height in Isd 37SC2 (4.71m), stalk height in Isd 37SC2 (3.04m), stalk girth in Isd 2-54SC2 (2.57cm), number of internode in Isd 37SC2 (30.23), internode length in Isd 40SC4 (12.28cm), leaf length in Isd 2-54 SC3 (1.60m), leaf breadth in Isd 2-54SC2 (5.35cm) and number of green leaf in Isd 17SC3 (15.57). In respect of biochemical analysis the highest purity percent was recorded in Isd 17SC1 (90.20). The highest brix (22%), the highest pol % cane (15.73) and the highest recovery (12.93%) were found in Isd 17SC4. These somaclones could be forwarded for the development of commercial variety.