Phytoremediation is a method of choice for remediates any pollutant from nature. This nascent technology has already undergone successful pilot study and it is time to find more popular and useful plants and bring them to the field to clear the nature. Alfalfa and tomato as a part of traditional culture of many countries could be proper candidates for this purpose. EtBr as a DNA staining dye is one of common tools of molecular biologist. But, the hazardous effects of them (in use or waste) make a new effort to remove safely them after usage. This study examine the alfalfa and tomato`s role in absorbance of EtBr and the subsequence of such accumulation. Seeds of alfalfa and tomato were transplanted and after 23 days in which they grown in appropriate size, we started to inoculate them with several concentration of EtBr. After 10th and 17th day the samples were collected and the analysis significantly demonstrated the diverse effect of EtBr on short and long term on them. In summary, EtBr divided the plants growth to two main phases, before day 10, increasing growth and after day 10, decreasing growth. Increasing roots number and diameter in contrast to decreasing the length of shoots were the special efforts of our research.