Anacardic acid (AA), has been described as potential antioxidant and Nrf2 is knowing as transcription factor responsible for synthesis of antioxidants proteins. The objective this study was verify the power antioxidant of AA, utilized frog palate epithelium as model of mucociliary epithelium and H2O2as potent oxidative specie:1) Control Group (CG) - Ringer’s solution (RS)/90’ (n=8); 2) H2 O2 (100 mM)/90’ (n=14; 3) AA (50mg/kg)/30’ and RS/90’ (n=4); 4) AA (50mg/kg)/30’ and H2 O2 (100 mM)/90’ (HA) (n=4). It was studied the mucociliary transport (MT), ciliary beat frequency (CBF), mucus profile (MP) and expression of Nrf2 (nucleus and cytoplasm). MT and CBF were evaluated through Two-Way ANOVA from two factors: time and treatment. MP and Nrf2 localization were evaluated by One-Way ANOVA test or Kruskal Wallis (p<0.05). MT was significant to time and treatment (p<0.001), CBF only treatment (p<0.001). MP was significant between AH and H2 O2 for acid mucus (p = 0.008) and vacuoles between H2 O2 and CG (p = 0.003). Nrf2 was different with relation nuclei between H2 O2 and CG (p=0.048) and H2 O2 and AH related with cytoplasm (p=0.003). AA showed to have a protective effect against reactive oxygen species without requirements of transcription factor Nrf2 translocate to nucleus.