Over the last decade non-native plants have been observed expanding their ranges in Ethiopia and a number of new non-native plants have been discovered. There is concern that with climate change invasive plants could cause ecological and economic damage like that seen in other parts of the world. In order to develop a strategy for combating invasive plants, a baseline inventory of Invasive Alien Plant Species (IAPS) in the study was required. Of all woredas five Woredas in Metekel Zone was selected based on pre-informed level of IAPS infestation, the assessment identified both infestation status and impact of IAPS. Four IAPS namely Parthenium hysterophorus, Lantana camara, Senna obtusifolia and Hyptis suaveolens that threatens native habitats along roadsides, rangelands, rivers and free areas in towns of BGRS. All respondents (100%) agreed about the invasiveness of these four species. Majority of the respondents (96.6%) agreed that the level of its invasion has been increasing from time to time. Majority of the respondents (61.7%) reported that Hyptis suaveolens and Senna obtusifolia has been causing different damage to the community such as minimize the growth of other plants, expand and compete to agricultural, grazing land and communal land, toxicity and health of livestock and also human. The patterns of distribution of these IAPS show that invasion is most pronounced in areas that have received recent road way construction and maintenance of all woredas in metekel zone. Furthermore, populations around bridges and culverts pose a risk to river ecosystems. We also recorded the presence and abundance of IAPS at rest stops, campgrounds, gravel pits and other disturbed areas along major roadsides of Metekel zone. As observed during the assessment frequently cultivated areas, highly grazed lands, frequently maintained roadways, market areas and roads which receive the most vehicle traffic had showed the highest infestation by invasive alien plants species.