An experiment containing callus induction has been conducted at the Laboratory of Breeding Division of Bangladesh Sugarcane Research Institute (BSRI) during the period of 2011 at BSRI farm, Ishurdi, Pabna. Leaf sheaths of small segments of young meristem of five varieties viz. Isd 2-54, LJ-C, Isd 17, Isd 37 and Isd 40 were used as explant. These segments were cultured in MS medium containing different concentrations of 2,4-D (viz. 1.0, 2.0, 3.0, 4.0 and 5.0 mg/l) for homogeneous callus induction. Varietal response on application of 2, 4-D, the variety Isd 17 showed the best results in callus induction. Among the five concentrations of 2, 4-D, the best response was exhibited in 3.0 mg/l of 2,4-D. Regarding interaction effect of varieties and doses of 2,4-D, the highest percentage (100%) of explants induced callus was recorded in variety Isd 17, Isd 40 and Isd 2-54 on 3.0 mg/l of 2,4-D respectively.