Glioblastoma (GBM) is an important tumor that has prevalence in childhood over adults, whereas many other types of tumor have high incidence after adolescence. It is a rare disease in terms of epidemiology of tumor incidence by age groups. The key role of synthetic endorphin in its RNA modification level is investigated in glioblastoma and neuroblastoma cell model by micro RNA array profiling. Our findings indicate that endorphins have highly skewed transcriptome processing profile in neuroblastoma as compared to glioblastoma. Also, cell viability decreased in Glioblastoma upon beta endorphin treatment. It provides a transcriptional model for endorphin neurotransmitter effects with two human brain derived cells.