Use of Bioinformatics Techniques in the Characterization of Genes and Proteins Involved in the Transport of Polyamines from Staphylococcus Genus - Abstract
Polyamines are essential nitrogenous compounds for bacteria, being captured by ABC transporters that recognize and interact with the molecule to be transported. Given the specificity of this interaction, the present work sought to identify genes and proteins of polyamine transporters in S. hyicus using bioinformatics tools. Sequence searches and tests suggest that S. hyicus possesses a spermidine ABC transporter, whose genes form the potABCD operon. From structural homology modeling it was pos sible to identify the specific amino acids in the interaction between PotD and spermidine where the W260 residue, characteristic of spermidine transporters, is predicted to be displaced into the pocket. This shift allows interaction with a minor polyamine such as putrescine whose transporter was not found in the S. hyicus genome. An in silico cloning of the PotD protein was carried out in the pET28a, pET28aSUMO, and pET44aNusA vectors and by submitting them for analysis of the resulting ORFs, strongly suggesting that they have favorable physicochemical qualities and solubility. For the docking, presentations present in the literature that were admired in past studies of polyamine transport inhibitors were selected. We can conclude that the S. hyicus PotABCD complex is a possible spermidine transporter and the inscriptions: AcSpm, NpAcSpm, GLT-Spm, DFMO, and MGBG, are possible competitive inhibitors of spermidine uptake in PotD in S. hyicus bacteria.