Bioautography Guided Isolation and Characterization of Antimicrobial Compounds of Picea smithiana - Abstract
The present study targeted to explore the antimicrobial constituents of Picea smithiana. It’s essential oil was analyzed by GC-MS and result showed that it has rich content of monoterpene compounds, and ?-pinene (38.82%), ?-pinene (7.41%), camphene (7.75%), Beta phellandrene (6.35%), a-bisabolol (5.60%), L-bornyl acetate (3.86%), Limonene (3.80%) and ?-salinene (3.30%) were the major components. The results showed that the essential oil exhibited good antibacterial activity against Micrococcus luteus, Bacillus subtilis and Pseudomonas alcaligenes and moderate activity against Alcalygens denitrificans, Campylobacter coli, Enterococcus fecalis and Pseudomonas aeruginosa with zone of inhibition ranging from 6-15mm and significant antifungal activity against Bipolaris specifera and Curvularia lunata with IC50 value 2.29mg/ml and 2.87mg/ml (w/v) respectively. Whereas, both leaf and bark extracts showed comparatively lower antimicrobial activity against most of the test organisms. Two antimicrobial compounds were isolated from Picea smithiana essential oil by using bioautography, preparative TLC methods and identified as alpha-selinene and alphabisabolol and GC-MS analysis.