Differential Expression of Proteins in Human Prostate Cancer: A MALDI-MS Based Imaging Study - Abstract
Histopathology is used for diagnosis of cancers based on analyzing the changes in cellular morphologies and tissue architecture associated with the disease. Early stages of
cancers might only be associated with minimal phenotypic changes in cellular morphologies that can result in false negative histopathological observations. However, at this stage there
might be detectable changes in the protein expression profiles of tissues. Imaging, an application of mass spectrometry, can potentially map the in situ spatial distribution of proteins
across tissue sections in a two-dimensional plot. In the present study, using Matrix Assisted Laser Desorption Ionisation – Mass Spectrometry (MALDI-MS) based imaging, we report
differential expressions of vinculin, ribonuclease T2 and 60 kDa heat shock protein in areas of human prostate tissue sections that were pathologically demarcated as malignant.
An independent quantitative proteomics analysis revealed that ribonuclease T2 and 60 kDa heat shock protein as significantly overexpressed by 22.26- and 6 folds respectively
in cancerous prostate tissues compared to benign. Based on our observations, we propose that ribonuclease T2 and 60 kDa heat shock protein might be developed as diagnostic
biomarkers for prostate cancer in future.