Organic Solvent Tolerant Lipase from Pseudomonas aeruginosa FW_SH-1: Purification and Characterization - Abstract
We have aimed to isolate a new strain which produces organic solvent tolerant lipase, the strain was designated as FW_SH-1, that was identified as a Pseudomonas sp. The extracellular lipase from Pseudomonas aeruginosa FW_SH-1 was purified by Q - sepharose anion exchange chromatography. A final specific activity of 572 U/mg was achieved with 26.12 fold purification and an overall recovery of 10.21%. The molecular weight of the purified lipase was found to be 66 kDa using SDS polyacrylamide gel electrophoresis. The optimum pH and temperature for the activity of the purified lipase was found to be 8.0 and 45°C. The purified lipase was stable in the presence of hydrophilic solvents such as methanol, ethanol and propanol. The activity of the purified lipase was stimulated in the presence of Ca2+, Mg2+, Na+, K+ and Tween 20.