Evaluation of the Gastroprotective and Antioxidant Effects of Caffeine and Caffeic Acid on EthanolInduced Gastric Ulcer - Abstract
Gastric ulcer (GU) is one of the most common disorders of the gastrointestinal tract. Among the nutritional aspects involved in GU trigger process we have the coffee intake. Caffeine (Caf) is one of the most known compounds of coffee, just as caffeic acid (CA) - a metabolic product from chlorogenic acids - and both have high antioxidant activity, which can protect the gastric mucosa, since oxidative stress can trigger the onset of GU. Because that, the aim of this study was to analyze the cytoprotective capacity of Caf and AC in different concentrations in ethanol-induced GU. For this, 56 male Wistar rats were subjected to GU induction and divided into 7 groups: saline 10 mL/kg; carbenoxolone 100 mg/kg; CA 25 mg/kg; CA 40 mg/kg; Caf 50 mg/kg; Caf 300 mg/kg and sham. All animals received the specific treatment, absolute ethanol and then were euthanized. At the end of the experiment, the stomachs were removed and submitted to macroscopic analysis by measurement of ulcerated area and antioxidant activity. The macroscopic analysis revealed significant differences for all treatments compared to vehicle, with Caf 300 and CA 40, showing the best cytoprotection. The biochemical analysis revealed increased levels of GSH, GPx and GR in CA 40 and higher activity of GPx in Caf 300. With our results, we demonstrated the gastroprotective activity of caffeine and caffeic acid, with best results in groups Caf 300 and CA 40 showing the decrease in GU area and the antioxidant potential mediated by GSH and GPx pathways.