Urinary Tract Infection (UTI) is among the most common bacterial infections and is considered a public health threat given the increasing rate of antibiotic resistance. Bacteria may change from their normal shape to spheroplasts when exposed to antibiotics at inappropriate dosages. Here, we discuss the case of a patient whose urine sediment examination revealed the development of these abnormal components. Appropriate training of laboratory professionals on the identification of spheroplasts and their clinical significance is important to support the medical specialist in choosing an appropriate antibiotic therapy. Thus, microscopic examination of urinary sediment, offers results beyond the screening level.