Titanium dioxide (TiO2) powder was synthesized by co-precipitation method with cetyltrimethylammonium bromide (CTAB) act as a surfactant. The prepared TiO2 sample was characterized by X-ray diffraction (XRD), Scanning Electron Microscopy (SEM) and UV-Vis absorption spectra to analysis the structural, morphological and optical properties studies were prepared TiO2 nanopowder was pasted on conducting surface of FTO glass slide by using doctor blade method with triton X-100 as a binder. Chlorophyll and betalain dye pigment extracted from tridax procumbens leaf and beetroot, which was used as a sensitizer for natural dye sensitized solar cell (NDSSC). The optical properties of the natural dyes were also investigated to aqueous lugol’s iodine was used as an electrolyte. The 1:1 ratio of Chlorophyll and betalain dye mixer was used as a sensitizer in NDSSC have cell efficiency of 0.11% was obtained under one sun solar simulator.