Association of Sperm DNA Fragmentation Level with Body Mass Index of Male and In Vitro Fertilization Outcomes: A Large-Scaled Retrospective Study - Abstract
Objective: To investigate the effect of male’s BMI on semen quality, embryo formation, development and pregnancy outcomes in couples undergoing in vitro fertilization-embryo transfer (IVF-ET) fresh cycle treatment.
Methods: The clinical data of 5,997 IVF-ET cycles that were performed between January 2018 and May 2020 were retrospectively analyzed. According to the BMI, these cycles were divided into Lean group (BMI < 18.5 Kg/m2), Normal group (18.5 Kg/m2 ? BMI < 24 Kg/m2), Overweight group (24 Kg/m2 ? BMI < 28 Kg/m2) and Obese group (BMI ? 28 Kg/m2). At the same time, the cycles were divided into the Low DFI group (DFI < 20%) and High DFI group (DFI ? 20%) according to sperm DNA fragmentation index (DFI). Then, the indicators of fertilization and (or) embryo development, embryo quality, pregnancy outcome were compared among the groups. Finally, logistic regression analysis was used to exclude the influence of female factor and other confounding factors in order to better study the effect of BMI of male on clinical pregnancy outcome.
Results: Progressive motility sperm percentage in Normal group was higher compared to Obese group, and the DFI in Overweight and Obese group was higher than that in Normal group (P<0.05). The rate of blastocyst formation in Lean group was higher than that in Obese group, while compared with Normal group, blastocyst formation rate in Overweight and Obese group was lower (P<0.05). Compared with Low DFI group, the blastocyst formation rate and high scoring blastocyst rate in High DFI group were lower(P<0.05). Abortion rates in Normal group were lower than that in Overweight and Obese group, while live birth rates were higher (P<0.05).
Results: Progressive motility sperm percentage in Normal group was higher compared to Obese group, and the DFI in Overweight and Obese group was higher than that in Normal group (P<0.05). The rate of blastocyst formation in Lean group was higher than that in Obese group, while compared with Normal group, blastocyst formation rate in Overweight and Obese group was lower (P<0.05). Compared with Low DFI group, the blastocyst formation rate and high scoring blastocyst rate in High DFI group were lower(P<0.05). Abortion rates in Normal group were lower than that in Overweight and Obese group, while live birth rates were higher (P<0.05).