Environmental endocrine disrupting chemicals (EEDCs) are widely present in people’s daily living environments. EEDCs can mimic endogenous hormones or competitively bind to their receptors, thereby exerting a series of biological functions and posing a serious threat to human reproductive health. Seeking the relationship between EEDCs and the abnormal zona pellucida (ZP) of the human oocyte and clinic treatment outcomes is of significant importance for shortening the time for women to prepare for pregnancy and maintaining human reproductive health. 19 cases of patients (case group) with oocytes exhibiting a waxy or indented ZP were retrospectively analyzed in our reproductive center. We found that patients in case group have significantly increased infertility duration and transfer cancellation rate, significantly reduced AMH level, E2 level on hCG day, average number of retrieved oocytes, oocyte maturation rate, fertilization rate and normal fertilization rate. Oocytes with waxy or indented ZP often experience all fertilization failure or low fertilization rates after conventional IVF fertilization, the fertilization problem can be resolved by ICSI fertilizaiton. There were no significant differences compared to the control group in clinical pregnancy rate (34.62% vs 47.06%), miscarriage rate (0 vs 4.71%) and live birth rate (34.62% vs 42.35%). 15 out of 19 patients had occupational exposure, such as the clothing/shoe sales or fabric dyeing industry, the makeup industry, the decoration industry, and the catering industry. Patients with oocytes exhibiting a waxy or indented ZP usually have a history of occupational exposure or long-term adverse environmental contact.