Background: The purpose of this study was to investigate the changes and relationship of the expression of miRNAs 34c (mir 34C) and miRNAs 15b (mir15b) with sperm parameters in infertile oligoasthenoteratozoospermia men during the sperm freezing-thawing process. Materials and Methods: In this experimental study, 25 semen samples were collected of oligoasthenoteratozoospermia men, who referred to infertility treatment Center Qom. Each sample was divided into two, non-frozen (Fresh) and frozen groups. Sperm parameters were evaluated using WHO criteria. Oxidative stress markers (ROS), total antioxidant capacity (TAC) and Malondialdehyde (MDA) level, were determined by ELISA kit. The expression level of miRNAs (34c and 15b) was assessed by Real-time PCR technique. Results: The expression of miRNAs 34c decreased and miRNAs 15b increased significantly in the frozen group compared to the fresh group (P<0.01). There was a significant decrease in motility, morphology, and viability in the frozen group compared to the fresh group (P<0.05). A significant increase in the level of ROS, MDA, and DNA fragmentation and a decrease in the level of TAC, were observed during the freeze-thaw process in oligoasthenoteratozoospermia (P<0.05). Sperm concentration, motility and morphology as well as oxidative stress factors and DNA integrity were correlated with the expression level of miRNAs (34c and 15b) (P<0.05). Conclusion: In summary, our study suggested that cryopreservation of sperm can change the expression of miRNAs (miR-34c, and miR15b) could impact fertility potential. In Addition, there was a meaningful correlation between microRNAs and sperm quality post freeze-thawing process in Oligoasthenoteratozoospermia men.