Expression and Differential Regulation of NR5A1 or SF1 in Harderian Gland of Hamster - Abstract
The nuclear receptor of subfamily 5 group A member 1 (NR5A1), also called steroidogenic factor 1 (SF1), is critical to the steroidogenesis process, controlling the expression
of cytochrome P450scc and other essential proteins for this process. There is evidence that the Harderian gland (HG) of the Syrian hamster (Mesocricettus auratus) expresses
steroidogenic enzymes. Some present a dimorphic expression, suggesting that sex steroids play a role in said expression. However, the expression of NR5A1/SF1 in HG has not been
identified, and whether this expression presents dimorphic profiles in HG and other steroidogenic tissues. In this study, part of the cDNA corresponding to NR5A1/SF1 was isolated,
and its experimental sequence was obtained, which will serve to obtain the complete cDNA sequence of this factor. So far, the cDNA’s central and 3’ region has been sequenced
and compared using the Multiple Sequence Alignment by CLUSTALW online tool, with the predicted sequence for the NR5A1/SF1 cDNA, obtaining an alignment score of 94.24%.