Objective: To investigate the therapeutic effect of CannaBiDiol (CBD) on OsteoArthritis (OA) in rats. Methods: We randomly divided 36 female SD rats with SPF values of 7 weeks into model control, CBD at elevated doses, CBD at low doses, solvent control, and PBS control. Sodium acetate OA was modeled in the model, experimental and solvent control groups. After modeling, the experimental group was injected daily with CBD and solvent, and the control group was injected with PBS. After 12 days of treatment, the OA grade was scored, and the rats were anesthetized, and the rats were sacrificed by neck dislocation, blood was collected from the heart, knee joints were collected, the degree of joint swelling was recorded, and serum inflammatory cytokines IFN-?, IL-1?, TFN-? and IL-6 concentration were and pathological changes of knee joint. Results: Compared with the model group and the solvent control group, the pain and claudication of the rats in the CBD experimental group were effectively relieved, and the levels of inflammatory cytokines (IFN-?, IL-1?, TFN-?) in serum and synovial fluid were significantly reduced. Arthritic pathologic changes in the knee were significantly reduced. Conclusion: CBD is effective in reducing blood inflammatory factors in OA rats, relieving pain and pathological changes, and has significant therapeutic effects in osteoarthritis.