Introduction: Aging processes are connected to chronic inflammation, known as “inflammaging”. Age-associated inflammation is a critical predictor of age-related morbidity and mortality in older adults, specifically serum Tumor Necrosis Factor alpha (TNF-alpha) and interleukin (IL-6) levels. Mesenchymal Stem Cells (MSCs) and their exosomes can alleviate systemic inflammatory processes. We present a simple technique for harvesting autologous, peripheral MSC from venous blood together with their exosomes and we present a strategy of an intramuscular stem cell deposit combined with a three-dimensional Hyaluronic Acid (HA) matrix as scaffold for delayed stem cell release as an anti-inflammatory treatment for individuals with chronic systemic inflammatory conditions as possible strategy against inflammaging. Material and Methods: Thirty milliliters (ml) venous whole blood are drawn from the cubital vein. By using a special centrifuge, 5 mL of peripheral MSC are harvested out of the venous whole blood together with 7 mL of MSC-derived exosomes and other anti-inflammatory autologous components. Then, we mix it with 88mg cross-linked hyaluronic acid (Monovisc, Anika Therapeutics, United States) that acts ad three-dimensional scaffold and as habitat for the MSCs and should lead to slowed release of the MSCs and their exosomes. After four weeks after injection of the 3D-stem cell deposit, serum levels of Interleukin-6 (IL-6), Tumor Necrosis Factor-Alpha (TNF-alpha), C-reactive Protein (CRP) were analyzed in 18 individuals with history of chronic systemic inflammatory diseases. Results: We report on 35 individuals receiving the 3D-stem cell deposit between October 2023 and September 2024. There were no serious adverse effects or complications related to the injection of the 3D-stem cell deposit. All subjects receiving the 3D-stem cell deposit reported improvement of their general well-being within one week after the injection. Especially painful circumstances related to osteoarthritis, headache (migraine), lower back pain, cervical pain related to disc herniation, but also other inflammation-caused circumstances (atopic dermatitis, psoriasis, thyroid eye disease) improved subjectively as reported by all the individuals. All inflammation serum parameters significantly improved (dropped) after 4 weeks in all of the eighteen individuals analyzed. Discussion: According to the results of our study and according to the fact that such pro-inflammatory parameters significantly decreased after administration of the 3D-stem cell deposit one can extrapolate the results suggesting lower mortality and extended lifespan after 3D-stem cell deposit administration. As a consequence and according to our data, the 3D-stem cell deposit might be an easy, effective and safe longevity concept by immunomodulation and by alleviating chronic inflammation.