Abstract Background: Imams who lead Islamic worship services daily are at higher risk of developing voice problems. The Imam trainees during the initial stages of training are inclined to exert more effort to deliver the readings with articulatory and acoustic precisions as novice, in order to expand their pitch, loudness range to improve their voice. Aim of the study: To investigate some acoustic parameters of voice (MF0, MI, MNHR, Jitter% Shimmer%) in Indian Imam trainees and check for any possible association of the acoustic findings with the results of the Voice Handicap Index (VHI-10). Method: 40 male participants in the age range of 18-23 years enrolled in the religious training program for Imams were recruited for the study. All of them were native speakers of Malayalam language. Self-rating questionnaire, the Voice Handicap Index (VHI-10) was administered on all the participants, followed by audio-recordings of sustained productions of the vowel /a/ in their habitual voice. The voice samples were recorded using PRAAT software in a quiet environment at the training institutes of the participants. Acoustic analysis was done using PRAAT software. Result: The findings indicated significant differences in mean noise to harmonic ratio (MNHR) between beginners and experienced trainees. Similarly, an increase in the frequency and amplitude perturbation measures, i.e., jitter%, shimmer% were noted, even though statistical significance was absent. Whereas Self-rating questionnaire (VHI-10) did not show any voice trend.