Background: In the realm of ART (Assisted Reproductive Technology), ICSI (IntraCytoplasmic Sperm Injection) has accomplished an essential achievement where its favorable outcome revolves around gametes quality. Several existing studies signified the correlation among SOQ (Sperm and oocyte quality) in ICSI outcome. However, precise classification of defects in gametes is lacking in the previous studies. Objective: The main objective of the proposed study is to examine whether gamete quality influences the BR (blastocyst rate) and FR (fertilization rate) in ICSI results. Methods: The presented study uses a Lenshooke AI device for identification of sperm defects and Hamilton laser software Image capturing System and Manual identification of oocyte abnormalities for quantification and categorization of sperm defects and oocyte abnormalities. It is analyzed with 53 ICSI cycles from January to December 2013, where the defects are categorized as high, moderate, and low. Results: The outcome of the proposed study signified that the lower gamete scores (< 4.5) are linked with greater ICSI success by attaining 64.84% blastocyst rate and 80.12% fertilization rates compared to greater gamete scores. Conclusions: This categorization of defects assists clinicians to understand how the quality of the gamete affects the embryo and fertilization development.