Some Biological Effects of Galactose-Specific Lectins from Prostate Tissue with Different Pathologies in Tumor Cells Model In vitro - Abstract
Abstract
Introduction: The biological effects of cytoplasmic galactose-specific lectins fraction isolated from unaffected, as well as post-operational pathological (benign hyperplasia,
BPH, high-grade intraepithelial neoplasia, HGPIN, tumor precursor, atypical adenomatous hyperplasia, AAH, and prostate adenocarcinoma, PC) prostate tissues were studied on
tumor cell lines (MEC1 lymphocytes cell line and Human prostate PC3-cell line).
Methods and Results: Cytosolic galactose-specific lectin fractions from prostate tissue with different diagnosis were purified by affinity chromatography. The lectin effects were
studied on cell viability by MTT reduction method and on apoptosis by flow cytometry method. All testified lectin fractions, except from AAH tissue, markedly reduced also the cell
viability in MEC1 and PC3 cell lines and fraction isolated from HGPIN and PC tissues in time dependent manner increased apoptosis in MEC1 line lymphocytes.
Conclusions: Cytosolic galactose-specific lectins fractions depend on the form of glandular tissue disease and also on the type of cell (lymphocytes, PC3, used in the research).
Our findings suggest that lectins isolated from HGPIN and PC tissues can have defensive anti-tumor properties in tumors cell - MEC1 line lymphocytes and prostate PC3 cell line.