Preliminary in vitro trial was conducted to assess the licicidal potentials of extracts of Allium sativum against poultry wing louse, Lipeurus caponis through filter paper bioassay. The ethanolic extract of A. saiivum exhibited lousicidal properties directly proportional to concentration and exposure time. The percentage mortality caused by 15 µLL-1 concentration was recorded as 90% in 48 hrs. Hence, A. sativum extract should be further screened (for separation of fraction of active ingredient and its molecular characterization) to develop a potent herbal licicide (to replace organic pesticide). ANOVA (one way) test has confirmed that concentration statistically significant effect and the measured response increases with both time and concentration.