Liver Fluke: Elisa Diagnosis in Goats in Pakistan - Abstract
Fascioliasis is an economically important parasitic disease of small ruminants mainly caused by a trematodes of the genus Fasciola. The most important species responsible for fascioliasis are Fasciola hepatica and Fasciola gigantica. The study was conducted for detection of fascioliasis in small ruminants through ELISA in Multan. A total of 100 samples were collected randomly for examination of GIT parasites especially for prevalence of fascioliasis. The animals under study were selected randomly without any discrimination of sex and age. During coprological parasitological examination of goats of district Multan, the total number of positive cases for Fasciola hepatica was 33/100 (33%) by sedimentation technique(s) and 19/100 (19%) by direct examination of the slides while 25/100 (25%) through floatation method were diagnosed respectively. The ELISA was performed on serum samples along with Copro-ELISA on the naturally infested animals. The ELISA gave promising results. It is recommended that there should be a routine procedure for the detection/ diagnosis of fascioliasis in goats through ELISA for good sustainable production practices in free range(s).