Background: Palliative care is an essential component of modern healthcare, aiming to improve the quality of life for patients with life-limiting illnesses and their families. Despite its recognized importance, integration into routine clinical practice remains limited, particularly in low- and middle-income settings. This study explored the knowledge, perceptions, barriers, and ethical challenges of healthcare professionals regarding palliative care. Methods: A cross-sectional survey was conducted among healthcare workers across multiple hospitals. A total of 447 participants completed a structured questionnaire addressing sociodemographic characteristics, knowledge and training in palliative care, perceptions toward its implementation, perceived barriers, strategies for improvement, and ethical considerations. Data were analyzed descriptively and presented in tables and graphs. Results: The majority of participants were nurses (57%), followed by physicians (30%), with most respondents aged between 30 and 39 years. Only 5.6% reported strong knowledge of palliative care, while 51.2% demonstrated average knowledge and 43.2% poor knowledge. Formal training was limited, with just 39.4% receiving prior palliative care education. Overall, 79.4% expressed positive perceptions toward palliative care, recognizing its value in enhancing patient outcomes. Major barriers identified included lack of training (51.5%), inadequate resources (41.4%), heavy workload (29.1%), and cultural/system constraints (32.6%). Strategies proposed to address these challenges included expanded training programs (60.4%), stronger policy support (40.3%), improved resource allocation (33.6%), and community awareness initiatives (26.8%). Ethical dilemmas were reported by 62.6% of respondents, highlighting the need for clearer guidelines and support systems. Familiarity with the WHO definition and availability of institutional training were significantly associated with more favorable perceptions of palliative care integration (p = .001 and p = .023, respectively), while lack of training predicted higher perceived barriers (p = .002). Regression models explained 6.8% of the variance in perceptions and 11.0% in barriers. Conclusion: Healthcare professionals demonstrated a generally positive attitude toward palliative care but lacked sufficient knowledge and training. Structural and cultural barriers remain significant obstacles to implementation. Addressing these gaps through education, policy support, and resource strengthening is essential for integrating palliative care into healthcare systems and ensuring dignified, patient-centered care.