Background: Maternal breast milk (BM) is the recommended form of enteral nutrition for term and preterm or very low birthweight (VLBW) infants with much evidence highlighting the benefits. Aim: To increase awareness among mothers regarding human milk banking in developing countries like Bangladesh. Method: A qualitative study through face to face interviewing can be conducted for exploring the mother’s knowledge, attitude and perceptions. Discussion: When a mother is unable or unwilling to breastfeed her child due to maternal illness, insufficient milk supply or other reasons, an alternative must be sought. Individual studies and systemic review show that human milk banking is preferable than infant formula. In view of the potential consequences of formula feeding, a suitable substitute should reasonably be pasteurized donated BM, if the mother’s own milk is unavailable or inadequate. Moreover, BM has been shown to increase gastric emptying and intestinal motility, enhance gastrointestinal growth and maturation, and increase tolerance of a full enteral feeding specially for preterm and VLBW infants, conceivably owing to the macronutrients in BM being more easily digested than formula. Conclusion: There is a limited data on human milk banking as well as awareness regarding this issue in developing country like Bangladesh. Further research and collaboration will be needed to achieve a consistent approach towards human milk banking and ensure the anticipated cost benefits eventuate.