VEGF-A-expressing cells were observed throughout the entire cross-section of the UC. The results of this study, in which VEGF-A was expressed, suggest that UCs may be able to contribute to angiogenesis even after long-term cryopreservation for 7 years. Cryopreservation affects tissue activity. Based on our results, the authors believe that UC can be a useful biomaterial in the clinical application of regenerative medicine.