Hyperbaric Oxygen Induced Vitamin D Receptor and ROS Responsive Genes: A Bioinformatic Analysis - Abstract
Background and Aim: Although reactive oxygen species (ROS) are often attributed to pathological outcomes, they do function as important modulators of many cellular processes involved in homeostasis and cell survival. Hyperbaric oxygen therapy (HBOT) initiates ROS production and signaling to promote angiogenesis, while limiting inflammatory and prothrombotic processes. In this study we set out to elucidate potential novel ROS-mediated, pro-angiogenic pathways.
Methods: Human umbilical cord endothelial cells (HUVECs) were subjected to standard HBOT parameters. HBOT-induced differential gene expression analyses were performed by coupling high-throughput RNA screening with bioinformatics.
Results: HBOT differentially regulated genes involved in a myriad of biological process, including a VD31A hydroxylase/Vitamin D Receptor (VDR) signaling cascade that is both ROS responsive and pro-angiogenic.
Conclusions: HBOT regulates ROS responsive angiogenic genes, potentially in a VDR dependent manner.