Method Overview of Cardiomyocytes Generation from Pluripotent Stem Cells - Abstract
Pioneering preclinical studies have demonstrated that cardiomyocytes generated from pluripotent stem cells (PSC-CMs) hold tremendous potential for the treatment of tissue loss and insufficiency (such as HF and MI) that result from heart disease. PSCs stand out as the one of the most promising candidates due to their ability to differentiate into CMs. However, methods for inducing differentiation have lagged behind and are still considered controversial because of the poor capacity for CM differentiation. Here, we introduce alternative methods in various disciplines that promote cardiac differentiation and review the current efforts underway to refine techniques for regenerative purposes. Growth factors, small chemical molecules, electrical stimulation, miRs, and Nanopattered (NP) engineering techniques are emerging as efficient and reproducible options for fine-tuning of desired cardiac lineage differentiation properties and therapeutic treatments for regeneration of tissue lost as a consequence of heart disease.