The Relevance of Using 3D Cell Culture, in Addition to 2D Monolayer Culture: The New Frontiers In vitro Models for Stem Cells and Cancer Drug Discovery - Abstract
While two-dimensional (2D) cell culture is still a dominant method in many biological studies, three-dimensional (3D) culture systems have gained significant attention in stem cell, cancer research, and tissue engineering. For the past few years, numerous examinations have shown the 3D cell culture advantages in offering more physiologically relevant data for in vivo trials compare to results collecting from 2D cultures. In this review, we discuss the challenges in developing an accurate in vitro model that mimics the complexity of living tissues in order to recapitulate the phenotype of cells within a tissue and their interaction with their microenvironment. The evolution and progression of some of the 3D cell culture such as microfluidic chambers, organ-on-chips as well as rolls and their features are another focal points of this review.