Lymphocyte Subpopulations in the Peripheral Blood, Mesenteric Lymphoid Nodes and Colon in Experimental Chronic Ulcerative Colitis - Abstract
In experimental chronic ulcerative colitis induced by dextran sulfate sodium
salt the morphological changes in mesenteric lymph nodes and the composition of
lymphocyte subpopulations measured by flow cytometry were studied. In chronic
ulcerative colitis the reactive changes in the mesenteric lymph nodes are characterized
by follicular hyperplasia and sinus reaction. In chronic ulcerative colitis the amount of
CD4+CD25+FOXP3+ regulatory T cells was increased in the peripheral blood, colon
and mesenteric lymph nodes. In the colon and mesenteric lymph nodes the amount
of CD19+ B cells was increased which is typical for adaptive immunity and chronic
inflammatory process. In the colon the amount of CD3+CD4+ T-helper was increased
and in the mesenteric lymph nodes the amount of CD3+CD8+ cytotoxic T-lymphocytes
was also increased.