Clinical Correlation of Clinical Stage of Carcinoma Colon with Tissue Expression of MMP 2, MMP 9 AND TIMP 2 - Abstract
Objectives: Metastasis of cancer is an intricate and well-coordinated interaction of numerous cellular and molecular factors. Matrix metalloproteinases are zinc dependent proteolytic enzymes that mediate destruction of extracellular matrix. MMP2 & 9 plays a major role in normal homeostasis at cellular level. These enzymes are often overexpressed in malignant tissues, resulting in degradation of collagen in basement membrane, leading to enhanced tumour growth, invasion and metastasis. This study is to find correlation between tissue expression of MMP 2, MMP 9 and TIMP 2 in cancinoma colon with stage of cancer, metastasis and survival. Methods: Prospective observational study of 33 patients with carcinoma colon who underwent surgical treatment. Full thickness biopsy of tumour tissue was examined for tissue expression MMP 2, 9 and TIMP 2 and its correlation with cancer stage, metastasis and survival. Results: Tissue expression of MMP 2, MMP 9 and TIMP 2 were significantly more than adjacent normal tissue. MMP 9 had significant correlation with tumour stage, but MMP 2 & TIMP 2 had no significant correlation. MMP 2, MMP 9 and TIMP 2 had no significant correlation with metastasis or survival. Conclusions: Matrix metalloproteinases are enzymes at cellular level which play a key role in tissue homeostasis. Even though tissue expression of tumour tissue is more than adjacent normal tissue, they did not predict the clinical outcome (metastasis, survival) of the patients.